Lv had no effect on body weight and food intake of mice compared to the HFD group. At the end of the experiment t12the weights of the high-fat diet-fed rats were slightly higher than those of the rats in the NC group, but no significant differences were observed among all the five groups, suggesting that PSE intake did not influence rat growth Supplementary Table S2.
The sequences of the primers used for this study are shown in Table S1. A recent epidemiological analysis in humans also found an association between diabetes prevalence and sugar availability [ 11 ]. Measuring C-peptide was preferred to measuring insulin for evaluating insulinemia, because it is more stable in blood and is not affected by haemolysis [ 31 ].
Although most patients with NAFLD have few or no symptoms, it is becoming an increasingly frequent cause of chronic liver damage because of its potential to progress to steatohepatitis, fibrosis, cirrhosis and even hepatocellular carcinoma. The gradient elution was carried out as follows: In addition to triacylglycerols, there are many micronutrients such as phenolic compounds, phytosterols and tocopherols presented in rapeseed oil.
Western blot analysis Total proteins from the liver and ileum were extracted according to a previously described method The aim of this study was to determine the effect of rapeseed oil fortified with these micronutrients on the atherosclerosis risk factors in rats fed a high-fat diet.
However, in most cases, a combination of excessive caloric intake and availability of energy-dense meals is thought to be the main contributor to obesity 2. In accord with these findings, these micronutrients naturally abundant in rapeseed oil have been reported to increase antioxidant status and lipid peroxidation in plasma [ 17 ] as well as in brain [ 30 ].
However, most of these micronutrients are removed in traditional processing technology currently used in the world extraction and refiningwhich will have an adverse effect on the hepatoprotective effect.
Notably, hyperuricemia is component of metabolic syndrome reflecting insulin resistance 17and is a common feature in NAFLD patients. China Corresponding author. Compared with the hepatocytes from the rats in the HF group, those from the rats in the PSEs intervention groups did not have large lipid vacuoles, the lipid droplet diameter, number of lipid droplets, and area covered by the lipid droplets were significantly lower, the boundaries between hepatocytes were more obvious, and it was possible to identify lobular structures.
These meant that although there was little cholesterol contained in the rodent diet in this study, inhibition of intestinal cholesterol absorption was still the main mechanism responsible for the cholesterol-lowering effect of phytosterols. Phytosterols are structurally similar to cholesterol but themselves are absorbed only in trace amounts [ 6 ], and for this reason, these compounds inhibit cholesterol absorption including recirculating endogenous biliary cholesterol which is a key step in cholesterol elimination [ 6 ].
However, following treatment with PSEs at the three doses, the liver color and surface morphology were greatly improved compared with the HF group. All these beneficial effects of these micronutrients might play important roles in preventing the initiation and development of fatty livers.
These molecules have the potential to diffuse from their sites of origin to reach distant intracellular and extracellular targets, thereby amplifying the effects of oxidative stress Our present study revealed that all doses of PSEs 0. Normoglycemia, slight hyperglycemia, and the development of type 2 diabetes have been reported with different diet regimes Table 1.
The development of overt diabetes in these animals is controversial. As a kind of potent lipid-soluble antioxidants, tocopherols are also effective against liver lipoperoxidation induced by high-fat diet [ 29 ]. The fat in the diet was provided by different rapeseed oils mentioned above.
· Effects of rapeseed oil fortified with micronutrients (polyphenols, tocopherols and phytosterols) on the level of IL-6 and CRP in plasma of rats fed a high-fat diet. RRO: the refined rapeseed oil group; L.
M- and H- FRRO: fortified refined rapeseed oil with low, middle and high contents of micronutrients justgohostelbraga.com by: · Methods. High-fat diet (HFD), high-fructose beverages (HF) or both (HFHF) were compared to rats fed with normal diet (ND) for 8 months to induce T2D and its metabolic, oxidative, and functional complications.
RESEARCH ARTICLE Efficacy of phytosterols and fish-oil supplemented high-oleic-sunflower oil rich diets in hypercholesterolemic growing rats Estefania Alsinaa, Elisa V.
Macria, Fima Lifshitzb, Clarisa Bozzinic, Patricia N. Rodrigueza, Patricia M.
Boyerc and. Rapeseed oil fortified with micronutrients reduces atherosclerosis risk factors in rats fed a high-fat diet. Phytosterols have been reported to exert hypocholesterolemic effect by Cited by: Group HS (High-fat Enriched Experimental): standard high-fat diet enriched with 2% of conjugated linoleic acid (CLA Tonalin®) and 2% de phytosterols (Vegapure®).
Download full-size image Fig. justgohostelbraga.com by: · At the end of the experiment (t 12), the weights of the high-fat diet-fed rats were slightly higher than those of the rats in the NC group, but no significant differences were observed among all Cited by: 8.